Anti-Claudin 4 antibody produced in rabbit
SIGMA/SAB4500433 - affinity isolated antibody
Synonym: CLD4; CLDN4; CPE- receptor; Claudin-4; Clostridium perfringens enterotoxin receptor
Product Type: Chemical
Immunohistochemistry Anti-Claudin 4: Cat. No. SAB4500433: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Claudin 4 antibody. Cells on the left were treated with the Anti-Claudin 4 antibody. Cells on the right (negative control) were treated with both Anti-Claudin 4 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Claudin 4: Cat. No. SAB4500433: Immunoblotting analysis of extracts from HeLa cells, using Anti-Claudin 4 antibody. The lane on the left was treated with the Anti-Claudin 4 antibody. The lane on the right (negative control) was treated with both Anti-Claudin 4 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 22 kDa |
| NCBI accession no. | O14493   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O14493 |
| Biochem/physiol Actions: | CLDN4 (claudin 4) is associated with epithelial differentiation, and is widely implicated in carcinomas. Its expression is considered to differentiate carcinoma with that of sarcoma. Claudin 4 expression is implicated in gastric cancer induced by Helicobacter pylori infection. It contributes to invasion, metastasis and prognosis of the disease. The CLDN4 gene is known to be overexpressed in a number of chemoresistant and metastatic cancer. Its localization to the tight junction prevents paracellular diffusion. Along with this, the extracellular loop interaction mediated by claudin 4 with the neighbouring cells makes it a differentiation marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The CLDN4 (claudin 4) gene is mapped to human chromosome 7q11.23. The encoded protein is an integral member, localized to tight junction. It is also expressed in the epithelial basolateral membrane. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Claudin 4. Immunogen Range: 160-209 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |