Anti-Claudin 3 antibody produced in rabbit
SIGMA/SAB4500435 - affinity isolated antibody
Synonym: CPE-R 2; CPE-receptor 2; Claudin-3; Clostridium perfringens enterotoxin receptor 2; Ventral prostate.1 protein homolog
Product Type: Chemical
Immunohistochemistry Anti-Claudin 3: Cat. No. SAB4500435: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Claudin 3 antibody. Cells on the left were treated with the Anti-Claudin 3 antibody. Cells on the right (negative control) were treated with both Anti-Claudin 3 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Claudin 3: Cat. No. SAB4500435: Immunofluorescence of HuvEc cells using Anti-Claudin 3 antibody. Cells on the left were treated with the Anti-Claudin 3 antibody. Cells on the right (negative control) were treated with both Anti-Claudin 3 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Claudin 3: Cat. No. SAB4500435: Immunoblotting analysis of extracts from HuvEc cells, using Anti-Claudin 3 antibody. The lane on the left was treated with the Anti-Claudin 3 antibody. The lane on the right (negative control) was treated with both Anti-Claudin 3 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 23 kDa |
| NCBI accession no. | O15551   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O15551 |
| Application: | Anti-Claudin 3, C-Terminal antibody has been used in immunofluorescence and western blotting. |
| Biochem/physiol Actions: | In submandibular gland, claudin-3 (CLDN3) controls paracellular permeability with the help of TNF-α(tumor necrosis factor α). High level expression of CLDN3 improves the malignant ability of colorectal cancer cells. It participates in the organization and activities of tight junctions (TJs). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Claudin 3 Antibody detects endogenous levels of total Claudin 3 protein. |
| General description: | Claudin-3 (CLDN3) is a tetraspanin transmembrane protein, that is mapped to human chromosome 7q11.23. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Claudin 3. Immunogen Range: 171-220 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |