Anti-CRHR2 antibody produced in rabbit
SIGMA/SAB4500466 - affinity isolated antibody
Synonym: CRF-R 2; CRF2; CRH-R 2; Corticotropin-
Product Type: Chemical
Immunofluorescence Anti-CRHR2: Cat. No. SAB4500466: Immunofluorescence of MCF7 cells using Anti-CRHR2 antibody. Cells on the left were treated with the Anti-CRHR2 antibody. Cells on the right (negative control) were treated with both Anti-CRHR2 antibody and the synthesized immunogen peptide.
Western Blotting Anti-CRHR2: Cat. No. SAB4500466: Immunoblotting analysis of extracts from COLO205/RAW264.7 cells, using Anti-CRHR2 antibody. The lane on the left was treated with the Anti-CRHR2 antibody. The lane on the right (negative control) was treated with both Anti-CRHR2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 47 kDa |
| NCBI accession no. | Q13324   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q13324 |
| Application: | Anti-CRHR2 antibody has been used in immunoprecipitation and western blotting .{6,7} |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | CRHR2 (corticotropin releasing hormone receptor 2) is involved in the relaxation of smooth muscle response and may affect the response of acute airway bronchodilator to short acting β2 agonist treatment of asthma. Signaling pathways of CRHR2 modulate the hypothalamic-pituitary-ad |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-CRHR2 antibody detects endogenous levels of total CRHR2 protein. |
| General description: | The CRHR2 (corticotropin releasing hormone receptor 2) gene is a member of G protein coupled receptors (GPCRs) family. It is located on human chromosome 7p21-p15. It has 12 exons and approximately 50,000 base pairs (Kbp). It has 3 isoforms, such as CRHR2α β and γ out of which, CRHR2γ is mostly expressed in the brain and CRHR2α 2β are expressed in the brain and smooth muscle. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CRHR2. Immunogen Range: 71-120 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |