Anti-DCT antibody produced in rabbit
SIGMA/SAB4500626 - affinity isolated antibody
Synonym: DCT; DT; L-dopachrome δ-isomerase; L-dopachrome tautomerase; Tyrosinase-related protein 2
Product Type: Chemical
Immunofluorescence Anti-DCT: Cat. No. SAB4500626: Immunofluorescence of A549 cells using Anti-DCT antibody. Cells on the left were treated with the Anti-DCT antibody. Cells on the right (negative control) were treated with both Anti-DCT antibody and the synthesized immunogen peptide.
Western Blotting Anti-DCT: Cat. No. SAB4500626: Immunoblotting analysis of extracts from K562 cells, using Anti-DCT antibody. The lane on the left was treated with the Anti-DCT antibody. The lane on the right (negative control) was treated with both Anti-DCT antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 59 kDa |
| NCBI accession no. | P40126   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 |
| Biochem/physiol Actions: | Dopachrome tautomerase (DCT) participates in the production of melanin in melanosomes and also in the detoxification of the melanin precursors. It restricts the destructions induced by chemotherapeutic agents like X-ray or UVB (ultraviolet B) and gamma irradiation in melanoma cells and reduces the susceptibility of cell to oxidative stress. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-DCT Antibody detects endogenous levels of total DCT protein. |
| General description: | Dopachrome tautomerase (DCT) is also termed as tyrosinase-related protein 2 (TYRP2). It is present in melanocytes, RPE (retinal pigment epithelium) cells and HaCaTs (basal keratinocytes). It is located on human chromosome 13q32. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DCT. Immunogen Range: 51-100 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |