Anti-Aggrecan (Cleaved-Asp369), N-Terminal antibody produced in rabbit
SIGMA/SAB4500662 - affinity isolated antibody
Synonym: CSPCP; CSPG1; aggrecan core protein; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
Product Type: Chemical
Immunohistochemistry Anti-Aggrecan (Cleaved-Asp369): Cat. No. SAB4500662: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-Aggrecan (Cleaved-Asp369) antibody. Cells on the left were treated with the Anti-Aggrecan (Cleaved-Asp369) antibody. Cells on the right (negative control) were treated with both Anti-Aggrecan (Cleaved-Asp369) antibody and the synthesized immunogen peptide.
Western Blotting Anti-Aggrecan (Cleaved-Asp369): Cat. No. SAB4500662: Immunoblotting analysis of extracts from Jurkat cells, treated with Etoposide 25uM 60′, using Anti-Aggrecan (Cleaved-Asp369) antibody. The lane on the left was treated with the Anti-Aggrecan (Cleaved-Asp369) antibody. The lane on the right (negative control) was treated with both Anti-Aggrecan (Cleaved-Asp369) antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 44 kDa |
| NCBI accession no. | P16112   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | proteolytically cleaved (Asp369) |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P16112 |
| Application: | Anti-Aggrecan (Cleaved-Asp369), N-Terminal antibody has been used in western blotting and immunostaining. |
| Biochem/physiol Actions: | Mutations in aggrecan results in autosomal dominant short stature with accelerated skeletal maturation. Mutation in the variable repeat region of the aggrecan gene (AGC1) leads to spondyloepiphyseal dysplasia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Aggrecan is a large aggregating chondroitin sulfate proteoglycan of the human cartilage. It is encoded by AGC1 (aggrecan) gene. It is located on human chromosome 15q26.1. |
| General description: | Anti-Aggrecan (Cleaved-Asp369) Antibody detects endogenous levels of fragment of activated Aggrecan (Cleaved-Asp369) protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Aggrecan. Immunogen Range: 320-369 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |