Anti-EFNA1 antibody produced in rabbit
SIGMA/SAB4500696 - affinity isolated antibody
Synonym: B61; ECKLG; EFL1; EPH-related receptor tyrosine kinase ligand 1; ephrin-A1
MDL Number: MFCD02096315
Product Type: Chemical
Immunofluorescence Anti-EFNA1: Cat. No. SAB4500696: Immunofluorescence of HeLa cells using Anti-EFNA1 antibody. Cells on the left were treated with the Anti-EFNA1 antibody. Cells on the right (negative control) were treated with both Anti-EFNA1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-EFNA1: Cat. No. SAB4500696: Immunoblotting analysis of extracts from 293/Jurkat cells, using Anti-EFNA1 antibody. The lane on the left was treated with the Anti-EFNA1 antibody. The lane on the right (negative control) was treated with both Anti-EFNA1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 23 kDa |
| NCBI accession no. | P20827   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P20827 |
| Application: | Anti-EFNA1 antibody produced in rabbit has been used in western blot analysis. |
| Biochem/physiol Actions: | EphrinA1 (EFNA1) and its primary receptor, Eph receptor 2 (EphA2) are associated with various cancers, such as urinary bladder carcinoma, breast cancer gastric cancer, glioma and malignant mesothelioma. Expression pattern of EFNA1 can be used as a potential prognostic marker in colorectal cancer (CRC) patients and other human malignancies. Experimental studies state that, EphrinA1 (EFNA1) can be involved in both tumor promotion and inhibition, based on whether ephrin-A1 is bound to the receptor in soluble or membrane bound form, respectively. Activation of EphA2, induces indirect growth signal by overcoming ‘contact inhibition’. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | EphrinA1 (EFNA1), also known as angiogenesis factor, is encoded by the gene mapped to human chromosome 1q21-22. The encoded protein belongs to subclass A of ephrin family. EFNA1 is localized to cell membrane via a glycosylphosphatidylinosi |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EFNA1. Immunogen Range: 66-115 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |