Anti-EGFR antibody produced in rabbit
SIGMA/SAB4500711 - affinity isolated antibody
Synonym: ERBB1; Epidermal growth factor receptor; Epidermal growth factor receptor precursor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
MDL Number: MFCD00162282
Product Type: Chemical
Immunohistochemistry Anti-EGFR: Cat. No. SAB4500711: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-EGFR antibody. Cells on the left were treated with the Anti-EGFR antibody. Cells on the right (negative control) were treated with both Anti-EGFR antibody and the synthesized immunogen peptide.
Western Blotting Anti-EGFR: Cat. No. SAB4500711: Immunoblotting analysis of extracts from 293 cells, treated with EGF 200ng/ml 30′, using Anti-EGFR antibody. The lane on the left was treated with the Anti-EGFR antibody. The lane on the right (negative control) was treated with both Anti-EGFR antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 134 kDa |
| NCBI accession no. | P00533   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P00533 |
| Biochem/physiol Actions: | Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor α (TGFα), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin and epigen. The gene encoding it is localized on human chromosome 7p11.2. Recombinant soluble human EGFR is a 621 amino acid glycoprotein comprising the extracellular domain of EGFR, and migrates at an apparent MW of 97.5kDa by SDS-PAGE analysis under reducing conditions. Anti-EGFR Antibody detects endogenous levels of total EGFR protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EGFR. Immunogen Range: 651-700 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |