Anti-APLF antibody produced in rabbit
SIGMA/SAB4500756 - affinity isolated antibody
Synonym: APFL; C2orf13; Xip1; aprataxin and PNK-like factor
Product Type: Chemical
Immunohistochemistry Anti-APLF: Cat. No. SAB4500756: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-APLF antibody. Cells on the left were treated with the Anti-APLF antibody. Cells on the right (negative control) were treated with both Anti-APLF antibody and the synthesized immunogen peptide.
Western Blotting Anti-APLF: Cat. No. SAB4500756: Immunoblotting analysis of extracts from HT-29 cells, using Anti-APLF antibody. The lane on the left was treated with the Anti-APLF antibody. The lane on the right (negative control) was treated with both Anti-APLF antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 56 kDa |
| NCBI accession no. | Q8IW19   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q8IW19 |
| Biochem/physiol Actions: | Aprataxin and PNKP-like factor (APLF) acts as a DNA repair factor. The forkhead-associated (FHA) domain of APLF helps to bind to the phosphorylated FHA domain binding motifs (FBMs) in XRCC1 and XRCC4. Other domains of APLF recruites APLF to sites of DNA damage. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Aprataxin and PNKP-like factor (APLF) is also called as PALF and Xip1. It is located in the nucleus. APLF has a forkhead-associated (FHA) domain and two unique poly (ADP-ribose)-binding zinc finger (PBZ) domains in its C-terminal region. This gene is mapped to human chromosome 2p13.3. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human APLF. Immunogen Range: 82-131 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |