Anti-PIP5K antibody produced in rabbit
SIGMA/SAB4500758 - affinity isolated antibody
Synonym: 1- phosphatidylinositol-
Product Type: Chemical
Immunofluorescence Anti-PIP5K: Cat. No. SAB4500758: Immunofluorescence of COS7 cells using Anti-PIP5K antibody. Cells on the left were treated with the Anti-PIP5K antibody. Cells on the right (negative control) were treated with both Anti-PIP5K antibody and the synthesized immunogen peptide.
Western Blotting Anti-PIP5K: Cat. No. SAB4500758: Immunoblotting analysis of extracts from HepG2 cells, using Anti-PIP5K antibody. The lane on the left was treated with the Anti-PIP5K antibody. The lane on the right (negative control) was treated with both Anti-PIP5K antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 237 kDa |
| NCBI accession no. | Q9Y2I7   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9Y2I7 |
| Biochem/physiol Actions: | Phosphatidylinositol 4-phosphate 5-kinase (PIP5K)/Phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE) protein plays a key role in phosphoinositide 5-kinase and protein kinase activity. It is essential for post-Golgi vesicle processing. Mutation in the PIKFYVE gene is associated with fleck corneal dystrophy (FCD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | PIKFYVE belongs to an evolutionarily ancient gene family of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2)enzymes. PIKFYVE gene is located on human chromosome 2q34. Anti-PIP5K antibody detects endogenous levels of total PIP5K protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PIP5K. Immunogen Range: 71-120 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |