Anti-HER2 antibody produced in rabbit
SIGMA/SAB4500789 - affinity isolated antibody
Synonym: C-erbB-2; Epidermal growth factor receptor-related protein; ErbB2; HER2; kinase ErbB2
MDL Number: MFCD06411882
Product Type: Chemical
Immunohistochemistry Anti-HER2: Cat. No. SAB4500789: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-HER2 antibody. Cells on the left were treated with the Anti-HER2 antibody. Cells on the right (negative control) were treated with both Anti-HER2 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-HER2: Cat. No. SAB4500789: Immunofluorescence of HeLa cells using Anti-HER2 antibody. Cells on the left were treated with the Anti-HER2 antibody. Cells on the right (negative control) were treated with both Anti-HER2 antibody and the synthesized immunogen peptide.
Western Blotting Anti-HER2: Cat. No. SAB4500789: Immunoblotting analysis of extracts from 293 cells, using Anti-HER2 antibody. The lane on the left was treated with the Anti-HER2 antibody. The lane on the right (negative control) was treated with both Anti-HER2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 137 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P04626  |
| Application: | Anti-HER2 antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Human epidermal growth factor receptor 2 (HER2) signaling pathway stimulates cell proliferation and survival in the majority of breast cancers. Thus, overexpression of this protein causes breast cancer. Heterodimer complex of HER2 and phosphatidylinositide 3-kinase (PI3K) is the most potent stimulator of the PI3K/AKT anti-apoptosis pathway. Increased expression of HER2 is also observed in ovarian, colorectal, pancreatic, endometrial and gastric cancers. Trastuzumab, an antibody, works by binding to a domain in the external domain of HER2.This domain is missing in p95, a truncated form of HER2, and hence these cancer cells show resistance to trastuzumab. HER2 protein can be used as a prognostic marker and as a therapeutic option for gynecologic cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-HER2 Antibody detects endogenous levels of total HER2 protein. |
| General description: | Human epidermal growth factor receptor 2 (HER2), or erb-b2 receptor tyrosine kinase 2 (ErbB2), is encoded by the gene mapped to human chromosome 17. The encoded protein belongs to the family of epidermal growth factors. HER2 membrane protein is characterized with a cysteine-rich extracellular ligand-binding domain, a hydrophobic membrane spanning region, and an intracellular tyrosine kinase domain. This protein does not have a ligand, and it either forms heterodimers with other family members or homodimer with itself when expressed at very high levels, to get activated. HER2 is expressed at low level in normal tissues, but at high level in breast cancer tissues. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HER2. Immunogen Range: 641-690 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |