Anti-Estrogen Receptor-α antibody produced in rabbit
SIGMA/SAB4500811 - affinity isolated antibody
Synonym: ER; ESR; ESR1; ESTR; ESTRA
MDL Number: MFCD02677942
Product Type: Chemical
Immunohistochemistry Anti-Estrogen Receptor-α: Cat. No. SAB4500811: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Estrogen Receptor-α antibody. Cells on the left were treated with the Anti-Estrogen Receptor-α antibody. Cells on the right (negative control) were treated with both Anti-Estrogen Receptor-α antibody and the synthesized immunogen peptide.
Western Blotting Anti-Estrogen Receptor-α: Cat. No. SAB4500811: Immunoblotting analysis of extracts from MCF7 cells, treated with Estradiol, using Anti-Estrogen Receptor-α antibody. The lane on the left was treated with the Anti-Estrogen Receptor-α antibody. The lane on the right (negative control) was treated with both Anti-Estrogen Receptor-α antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 66 kDa |
| NCBI accession no. | P03372   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P03372 |
| Biochem/physiol Actions: | Estrogen receptor alpha (ERα) is involved in immune and inflammatory response. Aberrations in ESR1 results in Mayer-Rokitansky-Küster-H |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Estrogen Receptor-α Antibody detects endogenous levels of total Estrogen Receptor-α protein. |
| General description: | Estrogen receptor alpha (ERα) is a member of the nuclear receptor superfamily. It is also known as ESR1 (estrogen receptor). It is located on human chromosome 6q25.1. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha. Immunogen Range: 71-120 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |