Anti-Estrogen Receptor-β antibody produced in rabbit
SIGMA/SAB4500814 - affinity isolated antibody
Synonym: ESR2; ESTRB; Estrogen receptor β; NR3A2
MDL Number: MFCD01323253
Product Type: Chemical
Immunofluorescence Anti-Estrogen Receptor-β: Cat. No. SAB4500814: Immunofluorescence of HeLa cells using Anti-Estrogen Receptor-β antibody. Cells on the left were treated with the Anti-Estrogen Receptor-β antibody. Cells on the right (negative control) were treated with both Anti-Estrogen Receptor-β antibody and the synthesized immunogen peptide.
Western Blotting Anti-Estrogen Receptor-β: Cat. No. SAB4500814: Immunoblotting analysis of extracts from HeLa cells, treated with EGF 200ng/ml 30′, using Anti-Estrogen Receptor-β antibody. The lane on the left was treated with the Anti-Estrogen Receptor-β antibody. The lane on the right (negative control) was treated with both Anti-Estrogen Receptor-β antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 59 kDa |
| NCBI accession no. | Q92731   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q92731 |
| Application: | Anti-Estrogen Receptor-βantibody has been used in western blotting .{42,43} |
| Biochem/physiol Actions: | Estrogen Receptor-β (ERβ) is involved in immune and inflammatory response. In females, estrogen receptor β (ESR2) is expected to play an important role in the progress of MDD (major depressive disorder). Aberrations in ESR2 gene result in bone mineral density variation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Estrogen Receptor-β (ERβ) is a member of the nuclear receptor superfamily. It has nine exons and is mapped to human chromosome 14q23.2. Anti-Estrogen Receptor- |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-beta. Immunogen Range: 71-120 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |