Anti-Factor VIII antibody produced in rabbit
SIGMA/SAB4500827 - affinity isolated antibody
Synonym: AHF; F8B; F8C; antihemophilic factor; coagulation factor VIII
Product Type: Chemical
Western Blotting Anti-Factor VIII: Cat. No. SAB4500827: Immunoblotting analysis of extracts from HuvEc cells, using Anti-Factor VIII antibody. The lane on the left was treated with the Anti-Factor VIII antibody. The lane on the right (negative control) was treated with both Anti-Factor VIII antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 267 kDa |
| NCBI accession no. | P00451   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P00451 |
| Biochem/physiol Actions: | Mutation in the factor VIII gene (F8) results in haemophilia A (HA). Higher levels of VIII:C (FVIII:C) leads to venous and arterial thromboembolism. The factor VIII serve as a procoagulant cofactor in the intrinsic blood coagulation pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Factor VIII Antibody detects endogenous levels of total Factor VIII protein. |
| General description: | Blood clotting factor VIII (FVIII), a nonenzymatic cofactor of activated factor IX (FIXa) is of ~330 kDa. It has three homologous A-domains, a unique B-domain and two C-domains. It is located on chromosome Xq28. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Factor VIII. Immunogen Range: 2161-2210 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |