Anti-MART-1 antibody produced in rabbit
SIGMA/SAB4500949 - affinity isolated antibody
Synonym: Antigen LB39-AA; Antigen SK29-AA; MART-1; Melan-A protein; Melanoma antigen recognized by T-cells 1
MDL Number: MFCD01633630
Product Type: Chemical
Immunohistochemistry Anti-MART-1: Cat. No. SAB4500949: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-MART-1 antibody. Cells on the left were treated with the Anti-MART-1 antibody. Cells on the right (negative control) were treated with both Anti-MART-1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-MART-1: Cat. No. SAB4500949: Immunoblotting analysis of extracts from NIH-3T3 cells, using Anti-MART-1 antibody. The lane on the left was treated with the Anti-MART-1 antibody. The lane on the right (negative control) was treated with both Anti-MART-1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 13 kDa |
| NCBI accession no. | Q16655   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q16655 |
| Biochem/physiol Actions: | MART-1 (melanoma antigen recognized by T cells) is essential for the activities of the melanosomal matrix protein PMEL17 (premelanosome protein)/GP100 and the maturation of melanosomes. It plays a key role in pigmentation. MART-1 plays an important role in the expression, stability, trafficking and processing of Pmel17. |
| Biochem/physiol Actions: | Melanoma-associated antigen recognized by T cells-1 (MART-1) has been shown to be essential for the functioning of glycoprotein-100 (gp100). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | MART-1 (melanoma antigen recognized by T cells) is also termed as Melan-A. It is a type III membrane protein with a molecular weight of 22-24 kDa. It is present in the endoplasmic reticulum (ER), trans-Golgi network (TGN) and in melanosomes. MART-1 is a melanocyte lineage protein. This gene is mapped to human chromosome 9p24.1. |
| General description: | Melanoma-associated antigen recognized by T cells-1 (MART-1) (also known as Melan-A) is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. It is a transmembrane protein which is hydrophobic in nature. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. The protein is also expressed in melanoma tumors. Anti-MART-1 Antibody detects endogenous levels of total MART-1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MART-1. Immunogen Range: 41-90 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |