Anti-Fibronectin 1 antibody produced in rabbit
SIGMA/SAB4500974 - affinity isolated antibody
Synonym: CIG; FINC; cold-insoluble globulin; fibronectin; fibronectin 1
Product Type: Chemical
Immunohistochemistry Anti-Fibronectin 1: Cat. No. SAB4500974: Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using Anti-Fibronectin 1 antibody. Cells on the left were treated with the Anti-Fibronectin 1 antibody. Cells on the right (negative control) were treated with both Anti-Fibronectin 1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Fibronectin 1: Cat. No. SAB4500974: Immunoblotting analysis of extracts from HeLa cells, using Anti-Fibronectin 1 antibody. The lane on the left was treated with the Anti-Fibronectin 1 antibody. The lane on the right (negative control) was treated with both Anti-Fibronectin 1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 262 kDa |
| NCBI accession no. | P02751   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P02751 |
| Application: | Anti-Fibronectin 1, C-Terminal antibody has been used in western blotting, cell staining and immunofluorescence. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Fibronectin 1 antibody detects endogenous levels of total Fibronectin 1 protein. |
| General description: | Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Fibronectin 1. Immunogen Range: 2337-2386 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |