Anti-Glucagon antibody produced in rabbit
SIGMA/SAB4501137 - affinity isolated antibody
Synonym: Glicentin; Glicentin-related polypeptide; Glucagon-like peptide 1(7-37); Glucagon-like peptide 1(GLP-1); Oxyntomodulin
MDL Number: MFCD00162325
Product Type: Chemical
Immunofluorescence Anti-Glucagon: Cat. No. SAB4501137: Immunofluorescence of NIH-3T3 cells using Anti-Glucagon antibody. Cells on the left were treated with the Anti-Glucagon antibody. Cells on the right (negative control) were treated with both Anti-Glucagon antibody and the synthesized immunogen peptide.
Western Blotting Anti-Glucagon: Cat. No. SAB4501137: Immunoblotting analysis of extracts from COS7 cells, using Anti-Glucagon antibody. The lane on the left was treated with the Anti-Glucagon antibody. The lane on the right (negative control) was treated with both Anti-Glucagon antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 20 kDa |
| NCBI accession no. | P01275   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P01275 |
| Application: | Anti-glucagon antibody has been used in immunostaining and immunohistochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Glucagon-like peptide-1 (GLP-1) increases glucose-dependent insulin secretion and terminates the liberation of glucagon. It acts as a physiological regulator of appetite and food intake. GLP-1 also helps to prevent the gastrointestinal movement and secretion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Glucagon antibody detects endogenous levels of total Glucagon protein. |
| General description: | Glucagon-like peptide-1 (GLP-1) is a gastrointestinal hormone. It is a 30-amino acid peptide, that is generated by the intestinal epithelial endocrine L-cells. it is mapped to human chromosome 2q24. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Glucagon. Immunogen Range: 61-110 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |