Anti-GFAP antibody produced in rabbit
SIGMA/SAB4501162 - affinity isolated antibody
Synonym: Glial fibrillary acidic protein
Product Type: Chemical
Immunohistochemistry Anti-GFAP: Cat. No. SAB4501162: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-GFAP antibody. Cells on the left were treated with the Anti-GFAP antibody. Cells on the right (negative control) were treated with both Anti-GFAP antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-GFAP: Cat. No. SAB4501162: Immunofluorescence of COS7 cells using Anti-GFAP antibody. Cells on the left were treated with the Anti-GFAP antibody. Cells on the right (negative control) were treated with both Anti-GFAP antibody and the synthesized immunogen peptide.
Western Blotting Anti-GFAP: Cat. No. SAB4501162: Immunoblotting analysis of extracts from COLO205 cells, using Anti-GFAP antibody. The lane on the left was treated with the Anti-GFAP antibody. The lane on the right (negative control) was treated with both Anti-GFAP antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 49 kDa |
| NCBI accession no. | P14136   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P14136 |
| Application: | Anti-GFAP, N-Terminal antibody has been used in immunocytochemical analysis , confocal and fluorescent microscopic imaging , immunostaining and western blotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Glial fibrillary acidic protein (GFAP) regulates intermediate filament network dynamics, cell morphology and focal adhesions.It helps in maintaining myelination and stability of CNS (central nervous system) white matter architecture. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-GFAP Antibody detects endogenous levels of total GFAP protein. |
| General description: | Glial fibrillary acidic protein (GFAP) is an intermediate-filament protein found in the astrocytes of CNS (central nervous system). It is located on human chromosome 17q21. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human GFAP. Immunogen Range: 11-60 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |