Anti-Connexin 43 antibody produced in rabbit
SIGMA/SAB4501174 - affinity isolated antibody
Synonym: CX43; CXA1; CXN-43; Connexin 43; Gap junction 43 kDa heart protein
Product Type: Chemical
Western Blotting Anti-Connexin 43: Cat. No. SAB4501174: Immunoblotting analysis of extracts from MCF-7/COS7/Mouse Heart, using Anti-Connexin 43 antibody. The lane on the left was treated with the Anti-Connexin 43 antibody. The lane on the right (negative control) was treated with both Anti-Connexin 43 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 43 kDa |
| NCBI accession no. | P17302   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P17302 |
| Application: | Anti-Connexin 43, C-Terminal antibody produced in rabbit has been used in flow cytometry. |
| Biochem/physiol Actions: | In rodents, connexin-43 (Cx-43) shows specific temporal patterns of expression during brain development. Experimental studies reveal that, connexin-43 expression increases in the brain during sheep ontogenesis.This protein might play a crucial role in neuronal migration as it resides in radial glial fibers which are closely associated with migrating neurons. It is also involved in transmembrane NAD+ transport. connexin-43, which is an element of gap junction, helps in cell–cell communication. Cx-43 is known to induce [Ca2+] i burst phenomenon in uterine artery endothelial cells (UAECs). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Connexin 43 belongs to the connexin protein family that forms gap junction. This protein is highly expressed in neurons and radial glia during development and in astrocytes in adult brains. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Connexin 43. Immunogen Range: 332-381 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |