Anti-Connexin 43 antibody produced in rabbit
SIGMA/SAB4501175 - affinity isolated antibody
Synonym: CX43; CXA1; CXN-43; Connexin 43; Gap junction 43 kDa heart protein
Product Type: Chemical
Immunohistochemistry Anti-Connexin 43: Cat. No. SAB4501175: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Connexin 43 antibody. Cells on the left were treated with the Anti-Connexin 43 antibody. Cells on the right (negative control) were treated with both Anti-Connexin 43 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Connexin 43: Cat. No. SAB4501175: Immunofluorescence of A549 cells using Anti-Connexin 43 antibody. Cells on the left were treated with the Anti-Connexin 43 antibody. Cells on the right (negative control) were treated with both Anti-Connexin 43 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Connexin 43: Cat. No. SAB4501175: Immunoblotting analysis of extracts from A549 cells, using Anti-Connexin 43 antibody. The lane on the left was treated with the Anti-Connexin 43 antibody. The lane on the right (negative control) was treated with both Anti-Connexin 43 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 43 kDa |
| NCBI accession no. | P17302   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P17302 |
| Biochem/physiol Actions: | Mutations in Connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Connexin 43 Antibody detects endogenous levels of total Connexin 43 protein. |
| General description: | Connexin 43 (Cx43) is also known as GJA1 (gap junction protein α 1) and belongs to a large family of homologous connexin proteins. It has an intracellular N-terminus, four transmembrane domains, two extracellular loops, one cytoplasmic loop and an intracellular C-terminus. It is located on human chromosome 6q22-q23. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Connexin 43. Immunogen Range: 333-382 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |