Anti-MYLIP antibody produced in rabbit
SIGMA/SAB4501317 - affinity isolated antibody
Synonym: Inducible degrader of the low-density lipoprotein receptor; E3 ubiquitin-protein ligase MYLIP; MIR; Myosin regulatory light chain-interacting protein
Product Type: Chemical
Immunohistochemistry Anti-MYLIP: Cat. No. SAB4501317: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-MYLIP antibody. Cells on the left were treated with the Anti-MYLIP antibody. Cells on the right (negative control) were treated with both Anti-MYLIP antibody and the synthesized immunogen peptide.
Western Blotting Anti-MYLIP: Cat. No. SAB4501317: Immunoblotting analysis of extracts from HeLa/Jurkat cells, using Anti-MYLIP antibody. The lane on the left was treated with the Anti-MYLIP antibody. The lane on the right (negative control) was treated with both Anti-MYLIP antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 49 kDa |
| NCBI accession no. | Q8WY64   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q8WY64 |
| Application: | Anti-MYLIP antibody produced in rabbit has been used in western blot analysis. |
| Biochem/physiol Actions: | MYLIP (myosin regulatory light chain interacting protein) controls the LDLR (low density lipoprotein receptor) activity and stability, which might change due to fluctuation in the intracellular cholesterol levels. Mylip is associated with brain development. It induces platelet-derived growth factor (PDGF) signaling in fibroblasts. Mylip is also called as inducible degrader of the LDL-R (Idol), as it leads to receptor degradation, especially in macrophages and liver. It might serve as a target for drug development in disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The MYLIP (myosin regulatory light chain interacting protein) gene is mapped to human chromosome 6p22.3. The encoded protein is an E3 ubiquitin ligase, expressed in all tissues including hippocampus and cerebellum. Anti-MYLIP antibody detects endogenous levels of total MYLIP protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MYLIP. Immunogen Range: 161-210 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |