Anti-CARD10 antibody produced in rabbit
SIGMA/SAB4501342 - affinity isolated antibody
Synonym: CARD-containing MAGUK protein 3; CARD10; CARMA3; Carma 3; Caspase recruitment domain-containing protein 10
Product Type: Chemical
Immunofluorescence Anti-CARD10: Cat. No. SAB4501342: Immunofluorescence of HeLa cells using Anti-CARD10 antibody. Cells on the left were treated with the Anti-CARD10 antibody. Cells on the right (negative control) were treated with both Anti-CARD10 antibody and the synthesized immunogen peptide.
Western Blotting Anti-CARD10: Cat. No. SAB4501342: Immunoblotting analysis of extracts from HeLa/293 cells, using Anti-CARD10 antibody. The lane on the left was treated with the Anti-CARD10 antibody. The lane on the right (negative control) was treated with both Anti-CARD10 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 115 kDa |
| NCBI accession no. | Q9BWT7   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9BWT7 |
| Biochem/physiol Actions: | CARD10 (Caspase recruitment domain-containing protein 10) helps to arrange signaling complexes at plasma membrane. It also serves as a molecular scaffold for the assembly of a B-cell CLL/lymphoma 10 (BCL10) signaling complex that activates NF-κB (nuclear factor-κB). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-CARD10 antibody detects endogenous levels of total CARD10 protein. |
| General description: | CARD10 (caspase recruitment domain-containing protein 10) is an important BCL10 (B-cell CLL/lymphoma 10) interactor. It is a member of membrane-associated guanylate kinase family. It is mapped to human chromosome 22q13.1. The CARD10 protein has 1032 amino acids with an estimated molecular weight of 116 kDa. It consists of five putative functional domains, an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal tripartite structure consisting of a PDZ (post synaptic density protein (PSD95), drosophila disc large tumor suppressor (Dlg1) and zonula occludens-1 protein) domain, an SH3 (SRC homology 3 domain) domain, and a GUK (guanylate kinases) domain with homology to guanylate kinase. In several adult tissues, including heart, kidney, and liver, it is expressed as a 4.4-kilobase transcript. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CARD10. Immunogen Range: 481-530 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |