Anti-ACC1 antibody produced in rabbit
SIGMA/SAB4501396 - affinity isolated antibody
Synonym: ACAC; ACACA; ACC-α; ACCA; Acetyl-CoA carboxylase 1
Product Type: Chemical
Immunohistochemistry Anti-ACC1: Cat. No. SAB4501396: Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Anti-ACC1 antibody. Cells on the left were treated with the Anti-ACC1 antibody. Cells on the right (negative control) were treated with both Anti-ACC1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-ACC1: Cat. No. SAB4501396: Immunoblotting analysis of extracts from NIH-3T3 cells, treated with PMA 125ng/ml 30′, using Anti-ACC1 antibody. The lane on the left was treated with the Anti-ACC1 antibody. The lane on the right (negative control) was treated with both Anti-ACC1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 265 kDa |
| NCBI accession no. | Q13085   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q13085 |
| Application: | Anti-ACC1 antibody produced in rabbit has been used in immunoblotting at a dilution 1:500 and immunohistochemistry (1:50 dilution). |
| Biochem/physiol Actions: | Acetyl-CoA carboxylase 1 (ACC1) mediates the carboxylation of acetyl-CoA to form malonyl-CoA in an ATP-dependent manner. It plays a key role in lipogenesis, and its inhibition is regarded as one of the ways to target fatty acid synthesis, especially in metabolic disorders and metabolic syndromes. Haploinsufficiency of ACC1 gene impacts regular fatty acid metabolism. This, in turn, may lead to pathologies associated with infantile encephalitic illness and seizures. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Acetyl-CoA carboxylase 1 (ACC1) is expressed in adipose tissue, liver, and lactating mammary gland. It is a cytosolic enzyme. The ACC1 gene is mapped to human chromosome 17q12. It comprises biotin carboxylase (BC), carboxyl transferase (CT), and biotin carboxyl carrier protein (BCCP) domains. The BC and CT domains are bridged together through an interaction domain (BT) and a non-catalytic central domain region (CD). The ACC1 gene encompasses three distinct promoter (PI, PII, and PIII) regions. |
| General description: | Anti-ACC1 Antibody detects endogenous levels of total ACC1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ACC1. Immunogen Range: 46-95 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |