Anti-APC antibody produced in rabbit
SIGMA/SAB4501438 - affinity isolated antibody
Synonym: APC; APC protein; Adenomatous polyposis coli protein; DP2.5; adenomatous polyposis of the colon gene
Product Type: Chemical
Immunohistochemistry Anti-APC: Cat. No. SAB4501438: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-APC antibody. Cells on the left were treated with the Anti-APC antibody. Cells on the right (negative control) were treated with both Anti-APC antibody and the synthesized immunogen peptide.
Western Blotting Anti-APC: Cat. No. SAB4501438: Immunoblotting analysis of extracts from COLO205 cells, using Anti-APC antibody. The lane on the left was treated with the Anti-APC antibody. The lane on the right (negative control) was treated with both Anti-APC antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 311 kDa |
| NCBI accession no. | P25054   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P25054 |
| Biochem/physiol Actions: | Adenomatous polyposis coli (APC) plays an important role in cell division, adhesion and migration. Mutations in APC gene is associated with colorectal carcinomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Adenomatous polyposis coli (APC) is a tumour suppressor gene. It is located on human chromosome 5q21. |
| General description: | Anti-APC Antibody detects endogenous levels of total APC protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human APC. Immunogen Range: 2794-2843 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |