Anti-GRP78 antibody produced in rabbit
SIGMA/SAB4501452 - affinity isolated antibody
Synonym: 78 kDa glucose-regulated protein; BiP; GRP-78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein
Product Type: Chemical
Immunohistochemistry Anti-GRP78: Cat. No. SAB4501452: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-GRP78 antibody. Cells on the left were treated with the Anti-GRP78 antibody. Cells on the right (negative control) were treated with both Anti-GRP78 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-GRP78: Cat. No. SAB4501452: Immunofluorescence of COS7 cells using Anti-GRP78 antibody. Cells on the left were treated with the Anti-GRP78 antibody. Cells on the right (negative control) were treated with both Anti-GRP78 antibody and the synthesized immunogen peptide.
Western Blotting Anti-GRP78: Cat. No. SAB4501452: Immunoblotting analysis of extracts from HuvEc/HepG2/HeLa cells, using Anti-GRP78 antibody. The lane on the left was treated with the Anti-GRP78 antibody. The lane on the right (negative control) was treated with both Anti-GRP78 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 72 kDa |
| NCBI accession no. | P11021   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P11021 |
| Biochem/physiol Actions: | Glucose/regulated protein 78 (GRP78) participates in stress-associated diseases. It also participates in protein biogenesis (protein transport into the endoplasmic reticulum (ER), protein folding and assembly and ER associated protein degradation), signal transduction and calcium homeostasis. |
| Biochem/physiol Actions: | GRP78 levels are elevated during various human cancers and tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Glucose/regulated protein 78 (GRP78) is a stress-associated protein. It is mapped to human chromosome 9q33.3. It is also known as HSPA5 (heat shock 70kDa protein 5) and immunoglobulin heavy chain binding protein (BiP). It is present in nucleated cells like thyroid, lung, smooth muscle, liver and several cells of the immune system. |
| General description: | The molecular weight of GRP78 is 78kDa. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human GRP78. Immunogen Range: 605-654 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |