Anti-HSP60 antibody produced in rabbit
SIGMA/SAB4501464 - affinity isolated antibody
Synonym: 60 kDa chaperonin; CH60; CPN60; HSP-60; Heat shock protein 60
Product Type: Chemical
Immunohistochemistry Anti-HSP60: Cat. No. SAB4501464: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-HSP60 antibody. Cells on the left were treated with the Anti-HSP60 antibody. Cells on the right (negative control) were treated with both Anti-HSP60 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-HSP60: Cat. No. SAB4501464: Immunofluorescence of HepG2 cells using Anti-HSP60 antibody. Cells on the left were treated with the Anti-HSP60 antibody. Cells on the right (negative control) were treated with both Anti-HSP60 antibody and the synthesized immunogen peptide.
Western Blotting Anti-HSP60: Cat. No. SAB4501464: Immunoblotting analysis of extracts from COLO205 cells, using Anti-HSP60 antibody. The lane on the left was treated with the Anti-HSP60 antibody. The lane on the right (negative control) was treated with both Anti-HSP60 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 61 kDa |
| NCBI accession no. | P10809   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P10809 |
| Application: | Anti-HSP60, C-Terminal antibody has been used in SDS/PAGE and western blot analysis. |
| Biochem/physiol Actions: | Hsp60 (heat shock protein family D member 1) helps to gather proteins that are imported into mitochondrial matrix. Overexpression of Hsp60 results in Behcet′s Disease (BD),Crohn′s disease, juvenile dermatomyositis (JDM), and juvenile idiopathic arthritis (JIA) and rheumatoid arthritis (RA). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-HSP60 antibody detects endogenous levels of total HSP60 protein. |
| General description: | Hsp60 (heat shock protein family D member 1) is a mitochondrial chaperone, located in the endoplasmic reticulum (ER). It is mapped to human chromosome 2q33.1. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HSP60. Immunogen Range: 511-560 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |