Anti-GPR109 antibody produced in rabbit
SIGMA/SAB4501488 - affinity isolated antibody
Synonym: G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; GPR109B; Nicotinic acid receptor 2
Product Type: Chemical
Immunofluorescence Anti-GPR109: Cat. No. SAB4501488: Immunofluorescence of MCF7 cells using Anti-GPR109 antibody. Cells on the left were treated with the Anti-GPR109 antibody. Cells on the right (negative control) were treated with both Anti-GPR109 antibody and the synthesized immunogen peptide.
Western Blotting Anti-GPR109: Cat. No. SAB4501488: Immunoblotting analysis of extracts from RAW264.7 cells, using Anti-GPR109 antibody. The lane on the left was treated with the Anti-GPR109 antibody. The lane on the right (negative control) was treated with both Anti-GPR109 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 44 kDa |
| NCBI accession no. | P49019   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 |
| Biochem/physiol Actions: | GPR109B (G-protein coupled receptor 109B) helps to carry out the activities of D-Phe and D-Trp on neutrophils. HCA3 receptors helps to activate ERK1/2 through PTX (Pertussis toxin)-sensitive Gi (inhibitory G) protein-dependent pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-GPR109 antibody detects endogenous levels of total GPR109 protein. |
| General description: | GPR109B (G-protein coupled receptor 109B) is also called as hydroxycarboxylic acid receptor 3 (HCA3) and HM74 (G-protein coupled receptor). It belongs to the subfamily of G-protein-coupled receptors (GPCR). GPR109B is expressed in adipose tissue, lung, spleen and peripheral blood leukocytes. It is mapped to human chromosome 12q24.31. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human GPR109. Immunogen Range: 285-334 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |