Anti-VEGFR2 antibody produced in rabbit
SIGMA/SAB4501645 - affinity isolated antibody
Synonym: FLK1; KDR; kinase insert domain receptor; protein-tyrosine kinase receptor Flk-1; vascular endothelial growth factor receptor 2
MDL Number: MFCD01092518
Product Type: Chemical
Immunohistochemistry Anti-VEGFR2: Cat. No. SAB4501645: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-VEGFR2 antibody. Cells on the left were treated with the Anti-VEGFR2 antibody. Cells on the right (negative control) were treated with both Anti-VEGFR2 antibody and the synthesized immunogen peptide.
Western Blotting Anti-VEGFR2: Cat. No. SAB4501645: Immunoblotting analysis of extracts from SK-OV3 cells, using Anti-VEGFR2 antibody. The lane on the left was treated with the Anti-VEGFR2 antibody. The lane on the right (negative control) was treated with both Anti-VEGFR2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 151 kDa |
| NCBI accession no. | P35968   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P35968 |
| Application: | Anti-VEGFR2 antibody produced in rabbit has been used in histochemical analysis of hydrogels injected subcutaneously. |
| Biochem/physiol Actions: | Vascular endothelial growth factor receptor 2 (VEGFR2) and its ligand VEGF play a vital role in angiogenesis and inflammation. It also facilitates autocrine stimulation of VEGF. VEGFR2 is implicated in progression of various types of cancers such as gliomas, malignant peripheral nerve sheath tumors, triple-negative breast cancer (TNBC) and gastrointestinal stromal tumors (GISTs). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-VEGFR2 Antibody detects endogenous levels of total VEGFR2 protein. |
| General description: | Vascular endothelial growth factor receptor 2 (VEGFR2), also known as kinase insert domain-containing receptor (KDR), is encoded by the gene mapped to human chromosome 4q12. The encoded protein belongs to the VEGF receptor family. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human VEGFR2. Immunogen Range: 917-966 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |