Anti-C-KIT antibody produced in rabbit
SIGMA/SAB4501648 - affinity isolated antibody
Synonym: CD117 antigen; Proto-oncogene tyrosine-protein kinase Kit; SCFR; c-kit
MDL Number: MFCD00282164
Product Type: Chemical
Western Blotting Anti-C-KIT: Cat. No. SAB4501648: Immunoblotting analysis of extracts from COS7/A549, using Anti-C-KIT antibody. The lane on the left was treated with the Anti-C-KIT antibody. The lane on the right (negative control) was treated with both Anti-C-KIT antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 109 kDa |
| NCBI accession no. | P10721   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P10721 |
| Biochem/physiol Actions: | KIT (proto-oncogene receptor tyrosine kinase) is considered to be an important hematopoietic growth factor receptor. It is known to exhibit tyrosine kinase activity that is associated with a variety of biologic processes, such as cell proliferation, survival, apoptosis, and motility. Downregulation of this gene is observed during the disease progression of multiple myeloma. Variation in the gene contributes to at least 2% of all melanomas and is most frequently observed in acral and mucosal melanomas. Mutations in KIT is associated with gastrointestinal stromal tumors. |
| Biochem/physiol Actions: | KIT (proto-oncogene) participates in cell signal transduction. It also participates in the progression of hematopoietic stem cells, melanocytes, germ cells, mast cells and interstitial cells of Cajal. Mutations in KIT gene results in gastrointestinal stromal tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-C-KIT Antibody detects endogenous levels of total C-KIT protein. |
| General description: | KIT (proto-oncogene) is a type III receptor tyrosine kinase (RTK). It codes for a tyrosine kinase receptor (CD117). It is located on human chromosome 4q12. |
| General description: | The KIT (proto-oncogene receptor tyrosine kinase) gene is mapped to human chromosome 4q12. It encodes for a transmembrane glycoprotein that belongs to the receptor tyrosine kinases subclass III family. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human c-Kit. Immunogen Range: 688-737 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |