Anti-Keratin 14 antibody produced in rabbit
SIGMA/SAB4501657 - affinity isolated antibody
Synonym: CK-14; Cytokeratin-14; K14; Keratin type I cytoskeletal 14
Product Type: Chemical
Immunohistochemistry Anti-Keratin 14: Cat. No. SAB4501657: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Keratin 14 antibody. Cells on the left were treated with the Anti-Keratin 14 antibody. Cells on the right (negative control) were treated with both Anti-Keratin 14 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Keratin 14: Cat. No. SAB4501657: Immunofluorescence of NIH-3T3 cells using Anti-Keratin 14 antibody. Cells on the left were treated with the Anti-Keratin 14 antibody. Cells on the right (negative control) were treated with both Anti-Keratin 14 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Keratin 14: Cat. No. SAB4501657: Immunoblotting analysis of extracts from NIH-3T3 cells, using Anti-Keratin 14 antibody. The lane on the left was treated with the Anti-Keratin 14 antibody. The lane on the right (negative control) was treated with both Anti-Keratin 14 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 51 kDa |
| NCBI accession no. | P02533   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P02533 |
| Biochem/physiol Actions: | CK14/ KRT14 (keratin 14) participates in the collective invasion of salivary adenoid cystic carcinoma (SACC). Mutations in KRT14 results in epidermolysis bullosa simplex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Keratin 14 Antibody detects endogenous levels of total Keratin 14 protein. CK14/ KRT14 (keratin 14) is one of the cytokeratin isotypes of human cells. It is the intermediate filament protein, which is the feature of epithelial cells. It is located in human chromosome 17q21. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Keratin 14. Immunogen Range: 1-50 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |