Anti-Keratin 15 antibody produced in rabbit
SIGMA/SAB4501658 - affinity isolated antibody
Synonym: CK-15; Cytokeratin-15; K15; Keratin type I cytoskeletal 15
Product Type: Chemical
Immunohistochemistry Anti-Keratin 15: Cat. No. SAB4501658: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Keratin 15 antibody. Cells on the left were treated with the Anti-Keratin 15 antibody. Cells on the right (negative control) were treated with both Anti-Keratin 15 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Keratin 15: Cat. No. SAB4501658: Immunoblotting analysis of extracts from COS7 cells, using Anti-Keratin 15 antibody. The lane on the left was treated with the Anti-Keratin 15 antibody. The lane on the right (negative control) was treated with both Anti-Keratin 15 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 49 kDa |
| NCBI accession no. | P19012   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P19012 |
| Application: | Anti-Keratin 15 antibody produced in rabbit has been used in: immunohistochemistry (1:100), immunofluorescence (1:100), immunostaining |
| Biochem/physiol Actions: | Keratin 15 (KRT15) participates in maintaining cytoplasmic stability. It acts as a marker for skin stem cells in the hair follicle bulge. KRT15 plays a major role in epidermal homeostasis. It is associated with tumorigenesis. KRT15 might serve as a biomarker in predicting the prognosis of breast invasive carcinoma (BRCA) patients. Overexpression of the KRT15 gene is seen in squamous-cell carcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Keratin 15 Antibody detects endogenous levels of total Keratin 15 protein. |
| General description: | Keratin 15 (KRT15)/cytokeratin 15, a type I keratin, belongs to the keratin (KRT) gene family. It is expressed in basal keratinocytes of stratified epithelium. KRT15 gene is located on human chromosome 17q21.2. Anti-Keratin 15 antibody detects endogenous levels of total Keratin 15 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Keratin 15. Immunogen Range: 1-50 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |