Anti-MDM2 antibody produced in rabbit
SIGMA/SAB4501849 - affinity isolated antibody
Synonym: Double minute 2 protein; HDM2; Oncoprotein Mdm2; Ubiquitin-protein ligase E3 Mdm2; p53-binding protein Mdm2
MDL Number: MFCD00282354
Product Type: Chemical
Immunofluorescence Anti-MDM2: Cat. No. SAB4501849: Immunofluorescence of NIH-3T3 cells using Anti-MDM2 antibody. Cells on the left were treated with the Anti-MDM2 antibody. Cells on the right (negative control) were treated with both Anti-MDM2 antibody and the synthesized immunogen peptide.
Western Blotting Anti-MDM2: Cat. No. SAB4501849: Immunoblotting analysis of extracts from COS7 cells, using Anti-MDM2 antibody. The lane on the left was treated with the Anti-MDM2 antibody. The lane on the right (negative control) was treated with both Anti-MDM2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 55 kDa |
| NCBI accession no. | Q00987   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q00987 |
| Application: | Anti-MDM2 antibody has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Murine double minute 2 (MDM2) is a negative regulator of the p53 tumor suppressor. It regulates cell cycle, cellular differentiation, cell fate determination, DNA repair and transcription. Epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells are induced by MDM2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-MDM2 antibody detects endogenous levels of total MDM2 protein. |
| General description: | Murine double minute 2 (MDM2) is an oncogenic protein. It is an E3 ubiquitin-protein ligase, that is mapped to human chromosome 12q15. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MDM2. Immunogen Range: 132-181 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |