Anti-MYF5 antibody produced in rabbit
SIGMA/SAB4501943 - affinity isolated antibody
Synonym: MYF5; myogenic factor 5
Product Type: Chemical
Immunohistochemistry Anti-MYF5: Cat. No. SAB4501943: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-MYF5 antibody. Cells on the left were treated with the Anti-MYF5 antibody. Cells on the right (negative control) were treated with both Anti-MYF5 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-MYF5: Cat. No. SAB4501943: Immunofluorescence of HeLa cells using Anti-MYF5 antibody. Cells on the left were treated with the Anti-MYF5 antibody. Cells on the right (negative control) were treated with both Anti-MYF5 antibody and the synthesized immunogen peptide.
Western Blotting Anti-MYF5: Cat. No. SAB4501943: Immunoblotting analysis of extracts from COS7 cells, using Anti-MYF5 antibody. The lane on the left was treated with the Anti-MYF5 antibody. The lane on the right (negative control) was treated with both Anti-MYF5 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 28 kDa |
| NCBI accession no. | P13349   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P13349 |
| Application: | Anti-MYF5 antibody produced in rabbit has been used in western blotting and immunohistochemistry. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Myogenic factor 5 (MYF5) regulates the determination of skeletal myoblasts during embryonic development. Inactivation of MYF5 in mice results in abnormal development of the ribs, which eventually leads to perinatal death. The gene encoding this protein is localized on human chromosome 12q21. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MYF5. Immunogen Range: 21-70 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |