Anti-MYPT1 antibody produced in rabbit
SIGMA/SAB4501949 - affinity isolated antibody
Synonym: MBS; Myosin phosphatase target subunit 1; PPP1R12A
MDL Number: MFCD04118624
Product Type: Chemical
Immunohistochemistry Anti-MYPT1: Cat. No. SAB4501949: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-MYPT1 antibody. Cells on the left were treated with the Anti-MYPT1 antibody. Cells on the right (negative control) were treated with both Anti-MYPT1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-MYPT1: Cat. No. SAB4501949: Immunoblotting analysis of extracts from NIH-3T3 cells, using Anti-MYPT1 antibody. The lane on the left was treated with the Anti-MYPT1 antibody. The lane on the right (negative control) was treated with both Anti-MYPT1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 115 kDa |
| NCBI accession no. | O14974   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O14974 |
| Biochem/physiol Actions: | Myosin phosphatase target subunit 1 (MYPT1) has a role in the modulation of the myosin light chain phosphatase through its multiple phosphorylation sites. It aids in the interaction of protein phosphatase-1 catalytic subunit δ (PP1C) with myosin filaments. MYPT1 acts as an antagonist of polo-like kinase-1 (PLK1) and has a role in regulating mitosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-MYPT1 Antibody detects endogenous levels of total MYPT1 protein. Myosin phosphatase target subunit 1 (MYPT1) is also known as protein phosphatase 1 regulatory subunit 12A (PPP1R12A). It is the regulatory subunit of the myosin light chain phosphatase. MYPT1 has a molecular weight of 130 kDa. The gene encoding this protein is localized on human chromosome 12q21.2. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MYPT1. Immunogen Range: 621-670 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |