Anti-HNRNP M antibody produced in rabbit
SIGMA/SAB4501954 - affinity isolated antibody
Synonym: HNRPM; heterogeneous nuclear ribonucleoprotein M
Product Type: Chemical
Immunohistochemistry Anti-HNRNP M: Cat. No. SAB4501954: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-HNRNP M antibody. Cells on the left were treated with the Anti-HNRNP M antibody. Cells on the right (negative control) were treated with both Anti-HNRNP M antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-HNRNP M: Cat. No. SAB4501954: Immunofluorescence of MCF7 cells using Anti-HNRNP M antibody. Cells on the left were treated with the Anti-HNRNP M antibody. Cells on the right (negative control) were treated with both Anti-HNRNP M antibody and the synthesized immunogen peptide.
Western Blotting Anti-HNRNP M: Cat. No. SAB4501954: Immunoblotting analysis of extracts from HT-29 cells, using Anti-HNRNP M antibody. The lane on the left was treated with the Anti-HNRNP M antibody. The lane on the right (negative control) was treated with both Anti-HNRNP M antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 77 kDa |
| NCBI accession no. | P52272   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P52272 |
| Biochem/physiol Actions: | HnRNP M (heterogeneous nuclear ribonucleoprotein M) is an RNA binding protein and participates in mRNA splicing mechanism. It is significantly involved in the formation of mature mRNAs from heterogeneous nuclear RNAs. The encoded protein acts as an important gene expression regulating factor. HnRNPM is involved in the activation of IRES (internal ribosomal entry site)-dependent translation of FGF1 (fibroblast growth factor 1). Thus, this protein is associated with myoblast differentiation. HNRNP M expression is associated with the invasion and metastasis events of tumor cells. Upregulation of HNRNP M is observed in breast cancer and human colorectal epithelial carcinogenesis. Therefore, HnRNP M may serve as an important biomarker for cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-HNRNP M Antibody detects endogenous levels of total HNRNP M protein. |
| General description: | HNRNP M (heterogeneous nuclear ribonucleoprotein M) gene is mapped to human chromosome 19p13.2. It codes for a nucleocytoplasmic shuttling RNA binding protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human hnRNP M. Immunogen Range: 11-60 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |