Anti-NF1 antibody produced in rabbit
SIGMA/SAB4501977 - affinity isolated antibody
Synonym: Neurofibromatosis-
MDL Number: MFCD02264160
Product Type: Chemical
Immunofluorescence Anti-NF1: Cat. No. SAB4501977: Immunofluorescence of HepG2 cells using Anti-NF1 antibody. Cells on the left were treated with the Anti-NF1 antibody. Cells on the right (negative control) were treated with both Anti-NF1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-NF1: Cat. No. SAB4501977: Immunoblotting analysis of extracts from HepG2 cells, using Anti-NF1 antibody. The lane on the left was treated with the Anti-NF1 antibody. The lane on the right (negative control) was treated with both Anti-NF1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 319 kDa |
| NCBI accession no. | P21359   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P21359 |
| Biochem/physiol Actions: | NF1 (neurofibromin 1) is a tumor suppressor gene and negatively regulates RAS (proto-oncogene, GTPase). Mutation in NF1 is strongly associated with the pathogenesis of melanoma and also causes neurofibromatosis type 1. Loss of NF1 allele results in benign neurofibromas in nonmyelinating Schwann cell and is associated with desmoplastic melanoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-NF1 Antibody detects endogenous levels of total NF1 protein. |
| General description: | NF1 (neurofibromin 1) gene is mapped to human chromosome 17q11.2. The protein encoded contains more than 2800 amino acids. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NF1. Immunogen Range: 1551-1600 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |