Anti-NF-κB p105 antibody produced in rabbit
SIGMA/SAB4501989 - affinity isolated antibody
Synonym: DNA-binding factor KBF1; EBP-1; NF-κ-B1 p84/NF-κ-B1 p98; NFKB1; NFkB-p50
Product Type: Chemical
Immunohistochemistry Anti-NF-κB p105: Cat. No. SAB4501989: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-NF-κB p105 antibody. Cells on the left were treated with the Anti-NF-κB p105 antibody. Cells on the right (negative control) were treated with both Anti-NF-κB p105 antibody and the synthesized immunogen peptide.
Western Blotting Anti-NF-κB p105: Cat. No. SAB4501989: Immunoblotting analysis of extracts from MDA-MB-435 cells, using Anti-NF-κB p105 antibody. The lane on the left was treated with the Anti-NF-κB p105 antibody. The lane on the right (negative control) was treated with both Anti-NF-κB p105 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 105 kDa |
| NCBI accession no. | P19838   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P19838 |
| Biochem/physiol Actions: | NF-κB (nuclear factor κ B subunit) is an important transcription factor that regulates the process of apoptosis, immune response and cell-growth control genes. It is associated with a number of human disorders including inflammatory diseases and cancers. NF-κB signaling cascade is known to be stimulated by many genotoxic stresses and also DNA damage. NF-κB is involved in bone metabolism. Downregulation of NF-κB mediates GPER (G-protein coupled estrogen receptor) specific agonist G-1 induced EMT (epithelial mesenchymal transition) suppression. Thereby, it prevents the motility of triple-negative breast cancer cells in vitro. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-NF-κB p105 Antibody detects endogenous levels of total NF-κB p105 protein. |
| General description: | The gene NFκB1 (nuclear factor κ B subunit 1) is mapped to human chromosome 4q24. It codes for NF-κB p105/p50 isoforms. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50. Immunogen Range: 304-353 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |