Anti-NNOS antibody produced in rabbit
SIGMA/SAB4502010 - affinity isolated antibody
Synonym: BNOS; Constitutive NOS; N-NOS; NC-NOS; bNOS
MDL Number: MFCD01092550
Product Type: Chemical
Immunofluorescence Anti-NNOS: Cat. No. SAB4502010: Immunofluorescence of HeLa cells using Anti-NNOS antibody. Cells on the left were treated with the Anti-NNOS antibody. Cells on the right (negative control) were treated with both Anti-NNOS antibody and the synthesized immunogen peptide.
Western Blotting Anti-NNOS: Cat. No. SAB4502010: Immunoblotting analysis of extracts from Raw264.7 cells, treated with INF 2500u/ml 10′, using Anti-NNOS antibody. The lane on the left was treated with the Anti-NNOS antibody. The lane on the right (negative control) was treated with both Anti-NNOS antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 160 kDa |
| NCBI accession no. | P29475   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P29475 |
| Application: | Anti-NNOS antibody produced in rabbit has been used in immunohistochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | NNOS (nitric oxide synthase 1) is responsible for the synthesis of NO (nitric oxide) as well as superoxide. Stress induces the activity of NNOS and generation of NO and results in the formation of nitrogen radicals. Elevation of nitrogen radical leads to intracellular protein damage and also induces impairment to mitochondrial transport chain components. This generally results in cellular energy deficiency. NNOS is known to regulate vasodilation in the forearm, in response to stress. Neuronal nitric oxide synthase normally functions to control the basal coronary blood flow. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Anti-NNOS antibody detects endogenous levels of total NNOS protein. |
| General description: | The NNOS (nitric oxide synthase 1) gene is mapped to human chromosome 12q24.22. Neuronal nitric oxide synthase is known to be expressed in skeletal muscles, the heart and the brain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human nNOS. Immunogen Range: 818-867 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |