Anti-INOS antibody produced in rabbit
SIGMA/SAB4502011 - affinity isolated antibody
Synonym: HEP-NOS; hepatocyte NOS; inducible; inducible NOS; nitric oxide synthase
Product Type: Chemical
Western Blotting Anti-INOS: Cat. No. SAB4502011: Immunoblotting analysis of extracts from NIH-3T3 cells, using Anti-INOS antibody. The lane on the left was treated with the Anti-INOS antibody. The lane on the right (negative control) was treated with both Anti-INOS antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 131 kDa |
| NCBI accession no. | P35228   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P35228 |
| Application: | Anti-INOS, N-Terminal antibody produced in rabbit has been used in western blotting and immunohistochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Upregulation of INOS (inducible nitric oxide synthase) is observed during infections, tissue injury, and inflammation. INOS is responsible to produce NO (nitric oxide), which controls blood flow by inducing vascular relaxation. NO acts as a neurotransmitter and can facilitate immune responses. NO can also bring about anti-thrombotic and anti-inflammatory effects in response to oxidative stress, hypoxia, inflammatory cytokines (TNF-α, IL-1β, IFN-γ), and lipopolysaccharide endotoxin. iNOS generates more NO than the neuronal and endothelial NOS. Unlike the two isoforms, iNOS is calcium independent. INOS might be associated with type 2 diabetes mellitus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-INOS antibody detects endogenous levels of total INOS protein. |
| General description: | The INOS (inducible nitric oxide synthase) gene is mapped to human chromosome 17q11.2. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human iNOS. Immunogen Range: 117-166 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |