Anti-ENOS antibody produced in rabbit
SIGMA/SAB4502013 - affinity isolated antibody
Synonym: Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; cNOS
MDL Number: MFCD00675266
Product Type: Chemical
Western Blotting Anti-ENOS: Cat. No. SAB4502013: Immunoblotting analysis of extracts from Jurkat cells, using Anti-ENOS antibody. The lane on the left was treated with the Anti-ENOS antibody. The lane on the right (negative control) was treated with both Anti-ENOS antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 133 kDa |
| NCBI accession no. | P29474   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P29474 |
| Application: | Anti-ENOS antibody produced in rabbit has been used in SDS-PAGE and western blotting. |
| Biochem/physiol Actions: | ENOS (endothelial nitric oxide synthase) is responsible for the production of nitric oxide. eNOS is known to stimulate vasodilation and regulate inflammation and myocyte function. Impairment of endothelium-dependent vasodilatation that is observed in acute hyperglycaemia condition is due to suppression of ENOS. The gene might be involved in lipolysis. Variation in ENOS might lead to Henoch-Schönlein purpura. ENOS polymorphism affects the NO concentration in plasma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-ENOS Antibody detects endogenous levels of total ENOS protein. |
| General description: | The ENOS (endothelial nitric oxide synthase) gene is mapped to human chromosome 7q36.1. ENOS is expressed predominantly in the endothelial cells. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human eNOS. Immunogen Range: 581-630 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |