Anti-PAK1 antibody produced in rabbit
SIGMA/SAB4502062 - affinity isolated antibody
Synonym: α-PAK; PAK-1; Serine/
MDL Number: MFCD03455286
Product Type: Chemical
Immunohistochemistry Anti-PAK1: Cat. No. SAB4502062: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PAK1 antibody. Cells on the left were treated with the Anti-PAK1 antibody. Cells on the right (negative control) were treated with both Anti-PAK1 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-PAK1: Cat. No. SAB4502062: Immunofluorescence of HeLa cells using Anti-PAK1 antibody. Cells on the left were treated with the Anti-PAK1 antibody. Cells on the right (negative control) were treated with both Anti-PAK1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-PAK1: Cat. No. SAB4502062: Immunoblotting analysis of extracts from 293 cells, treated with Etoposide 25uM 60′, using Anti-PAK1 antibody. The lane on the left was treated with the Anti-PAK1 antibody. The lane on the right (negative control) was treated with both Anti-PAK1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 60 kDa |
| NCBI accession no. | Q13153   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q13153 |
| Biochem/physiol Actions: | PAK1 (p21 (RAC1) activated kinase 1) maintains the cytoskeleton and cellular morphology. It is associated with cell cycle, cell motility, survival, and cell death. PAK1 is upregulated in different types of cancer. PAK1 is found to be involved in growth, invasion, and metastasis of cancer cells, as it affects the action of pro-apoptotic signals. In neurodegenerative diseases, such as Huntington’s disease and Alzheimer’s disease, and mental retardation, abnormal regulation of PAK1 is observed. PAK1 induces MAPK (mitogen activated protein kinase) signal pathway and cytoskeletal remodeling in breast cancer, thereby serving as an oncogene. PAK1 is known to significantly participate in the development, maturation and plasticity of the nervous system. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-PAK1 antibody detects endogenous levels of total PAK1 protein. |
| General description: | The PAK1 (p21 (RAC1) activated kinase 1) gene is mapped to human chromosome 11q13.5-q14.1. It belongs to the family of serine/threonine kinases and the gene is evolutionarily conserved. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PAK1. Immunogen Range: 178-227 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |