Anti-PI3-kinase p85-α antibody produced in rabbit

SIGMA/SAB4502195 - affinity isolated antibody

Synonym: GRB1; P85A; PI3-kinase p85-α subunit; PI3K; PI3K p85-α

MDL Number: MFCD02677933
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SAB4502195-100UG	100 µg	$560.00 1/EA	Add To Favorites

Immunohistochemistry Anti-PI3-kinase p85-α: Cat. No. SAB4502195: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-PI3-kinase p85-α antibody. Cells on the left were treated with the Anti-PI3-kinase p85-α antibody. Cells on the right (negative control) were treated with both Anti-PI3-kinase p85-α antibody and the synthesized immunogen peptide.

Immunofluorescence Anti-PI3-kinase p85-α: Cat. No. SAB4502195: Immunofluorescence of HeLa cells using Anti-PI3-kinase p85-α antibody. Cells on the left were treated with the Anti-PI3-kinase p85-α antibody. Cells on the right (negative control) were treated with both Anti-PI3-kinase p85-α antibody and the synthesized immunogen peptide.

Western Blotting Anti-PI3-kinase p85-α: Cat. No. SAB4502195: Immunoblotting analysis of extracts from COS7 cells, treated with H2O2 100uM 30′, using Anti-PI3-kinase p85-α antibody. The lane on the left was treated with the Anti-PI3-kinase p85-α antibody. The lane on the right (negative control) was treated with both Anti-PI3-kinase p85-α antibody and the synthesized immunogen peptide.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	affinity isolated antibody
antibody product type	primary antibodies
biological source	rabbit
clone	polyclonal
concentration	~1 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
mol wt	antigen 83 kDa
NCBI accession no.	P27986
Quality Level	100
shipped in	wet ice
species reactivity	mouse, rat, human
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	ELISA: 1:1000
	immunofluorescence: 1:100-1:500
	immunohistochemistry: 1:50-1:100
	western blot: 1:500-1:1000
UniProt accession no.	P27986

Application:	Anti-PI3-kinase p85-α antibody produced in rabbit has been used in western blotting.
Biochem/physiol Actions:	PIK3R1 (phosphoinositide-3-kinase regulatory subunit 1) is known to mediate immune cell differentiation, development and function. Dominant mutations of PIK3R1 causes hyperactivation of the PI3K signaling pathway, leading to immunodeficiency and also SHORT syndrome (short stature, hyperextensibility, hernia, ocular depression, Rieger anomaly, and teething delay). PI3K is an important part of insulin and growth factor signaling. PIK3R1 gene is considered to be intolerant to functional variation among the human population. In human, downregulation of PIK3R1 is observed in many types of cancer. PIK3R1 is regarded as a tumor suppressor gene. PIK3R1 mediates tumorigenesis and malignant progression. The gene harbors receptor tyrosine kinases activity and participates in the activation of class IA PI3Ks.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits:	Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description:	Anti-PI3-kinase p85-α antibody detects endogenous levels of total PI3-kinase p85-α protein.
General description:	The PIK3R1 (phosphoinositide-3-kinase regulatory subunit 1) gene is mapped to human chromosome 5q13.1. It encodes for p85α regulatory subunit.
Immunogen:	The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma. Immunogen Range: 436-485
Physical form:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203

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