Anti-PLK1 antibody produced in rabbit
SIGMA/SAB4502211 - affinity isolated antibody
Synonym: PLK-1; Polo-like kinase 1; STPK13; Serine/
MDL Number: MFCD01634025
Product Type: Chemical
Immunofluorescence Anti-PLK1: Cat. No. SAB4502211: Immunofluorescence of A549 cells using Anti-PLK1 antibody. Cells on the left were treated with the Anti-PLK1 antibody. Cells on the right (negative control) were treated with both Anti-PLK1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-PLK1: Cat. No. SAB4502211: Immunoblotting analysis of extracts from LOVO cells, treated with PMA 125ng/ml 30′, using Anti-PLK1 antibody. The lane on the left was treated with the Anti-PLK1 antibody. The lane on the right (negative control) was treated with both Anti-PLK1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 68 kDa |
| NCBI accession no. | P53350   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P53350 |
| Biochem/physiol Actions: | PLK1 (polo like kinase 1) is predominantly involved in mitosis. It mediates the phosphorylation of cyclin B1/CDK1 (cyclin dependent kinase 1) complex, and activation of the anaphase promoting complex. PLK1 has anti-apoptotic function. Upregulation of gene is observed in different types of cancer, such as colorectal cancer and is regarded as an oncogene. PLK1 is involved in checkpoint recovery and adaptation following DNA damage. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-PLK1 Antibody detects endogenous levels of total PLK1 protein. |
| General description: | PLK1 (polo like kinase 1) gene is mapped to human chromosome 16p12.2. It codes for the protein serine/threonine kinase. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PLK1. Immunogen Range: 176-225 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |