Anti-Cytochrome c antibody produced in rabbit
SIGMA/SAB4502234 - affinity isolated antibody
Synonym: CYC; CYCS; Cytochrome c
Product Type: Chemical
Immunohistochemistry Anti-Cytochrome c: Cat. No. SAB4502234: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytochrome c antibody. Cells on the left were treated with the Anti-Cytochrome c antibody. Cells on the right (negative control) were treated with both Anti-Cytochrome c antibody and the synthesized immunogen peptide.
Western Blotting Anti-Cytochrome c: Cat. No. SAB4502234: Immunoblotting analysis of extracts from COS7 cells, using Anti-Cytochrome c antibody. The lane on the left was treated with the Anti-Cytochrome c antibody. The lane on the right (negative control) was treated with both Anti-Cytochrome c antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 11 kDa |
| NCBI accession no. | P99999   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P99999 |
| Application: | Anti-Cytochrome c, N-terminal antibody produced in rabbit has been used in western blot analysis. |
| Biochem/physiol Actions: | Cytochrome c is a water-soluble intermediary of electron transport chain. It acts as an ROS (reactive oxygen species) scavenger, and involved in redox-mediated protein import and apoptosis. Cytochrome c has peroxidase activity, contributing towards intrinsic pathway of apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Cytochrome c antibody detects endogenous levels of total Cytochrome c protein. |
| General description: | The cytochrome c gene is mapped to human chromosome 7p15.3. The encoded protein consists of two thioether present between heme and two cysteine residues included in a CXXCH motif (highly conserved). It is localized to mitochondrial intermembrane space. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Cytochrome c. Immunogen Range: 10-59 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |