Anti-Keratin 20 antibody produced in rabbit
SIGMA/SAB4502249 - affinity isolated antibody
Synonym: CK-20; Cytokeratin-20; K20; Keratin type I cytoskeletal 20
Product Type: Chemical
Immunohistochemistry Anti-Keratin 20: Cat. No. SAB4502249: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Keratin 20 antibody. Cells on the left were treated with the Anti-Keratin 20 antibody. Cells on the right (negative control) were treated with both Anti-Keratin 20 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Keratin 20: Cat. No. SAB4502249: Immunoblotting analysis of extracts from HeLa cells, using Anti-Keratin 20 antibody. The lane on the left was treated with the Anti-Keratin 20 antibody. The lane on the right (negative control) was treated with both Anti-Keratin 20 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| NCBI accession no. | P35900   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P35900 |
| Biochem/physiol Actions: | KRT20 (keratin 20) helps in intermediate filament organization. In metastatic colorectal cancer, it can be used as a biomarker for checking circulating tumor cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Keratin 20 Antibody detects endogenous levels of total Keratin 20 protein. The gene KRT20 (keratin 20) is mapped to human chromosome 17. The gene encodes an intermediate filament which is mainly expressed in the gastrointestinal epithelial cells and epidermal Merkel cells. The protein is a type 1 keratin. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Keratin 20. Immunogen Range: 375-424 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |