Anti-OCT3 antibody produced in rabbit
SIGMA/SAB4502259 - affinity isolated antibody
Synonym: OCT3; OCT4; OCTAMER-BINDING TRANSCRIPTION FACTOR 3; OTF3; OTF4
MDL Number: MFCD02263853
Product Type: Chemical
Immunohistochemistry Anti-OCT3: Cat. No. SAB4502259: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-OCT3 antibody. Cells on the left were treated with the Anti-OCT3 antibody. Cells on the right (negative control) were treated with both Anti-OCT3 antibody and the synthesized immunogen peptide.
Western Blotting Anti-OCT3: Cat. No. SAB4502259: Immunoblotting analysis of extracts from HeLa cells, using Anti-OCT3 antibody. The lane on the left was treated with the Anti-OCT3 antibody. The lane on the right (negative control) was treated with both Anti-OCT3 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 38 kDa |
| NCBI accession no. | Q01860   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q01860 |
| Application: | Anti-OCT3 antibody produced in rabbit may be used in immunohistochemistry at 1:100 dilution and immunoblot at 1:1000 dilution. |
| Biochem/physiol Actions: | Octamer-binding transcription factor 3 (OCT3)/OCT4 plays a vital role in the self-renewal and pluripotency of embryonic stem cells. It is involved in the regulation of embryonic development and cell fate determination. Overexpression of OCT4 gene aids in the progression of various types of human cancers by increasing tumorigenicity and enhancing resistance to chemotherapeutics. OCT4 expression acts as a potential cancer diagnosis and prognosis biomarker. Reduced expression of the OCT4 gene suppresses the growth of pancreatic cancer cells by blocking the AKT pathway. Therefore, OCT4 protein may be used as an effective therapeutic target for pancreatic cancer treatment. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-OCT3 Antibody detects endogenous levels of total OCT3 protein. |
| General description: | Octamer-binding transcription factor 3 (OCT3)/OCT4 is encoded by the gene spanning 1420 bases of genomic DNA mapped to human chromosome 6p21.33. OCT3/OCT4 is a transcription factor that belongs to the transcription Pit-Oct-Unc (POU)family. This protein contains a POU-specific homeodomain and DNA-binding domain. Anti-OCT3 antibody detects endogenous levels of total OCT3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human OCT3. Immunogen Range: 191-240 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |