Anti-AMPK α antibody produced in rabbit
SIGMA/SAB4502329 - affinity isolated antibody
Synonym: 5′-AMP-activated protein kinase; AAPK1; AMPK α-1 chain; AMPK-α1; catalytic α-1 chain
MDL Number: MFCD07370254
Product Type: Chemical
Immunohistochemistry Anti-AMPK α: Cat. No. SAB4502329: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-AMPK α antibody. Cells on the left were treated with the Anti-AMPK α antibody. Cells on the right (negative control) were treated with both Anti-AMPK α antibody and the synthesized immunogen peptide.
Western Blotting Anti-AMPK α: Cat. No. SAB4502329: Immunoblotting analysis of extracts from COS7 cells, treated with Adriamycin 0.5ng/ml 24h, using Anti-AMPK α antibody. The lane on the left was treated with the Anti-AMPK α antibody. The lane on the right (negative control) was treated with both Anti-AMPK α antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 62 kDa |
| NCBI accession no. | Q13131   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 |
| Application: | Anti-AMPK α antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | The AMPK α (protein kinase AMP-activated catalytic subunit α1) gene is associated with the regulation of cell cycle, cell proliferation, energy metabolism and inhibition of rapamycin-pathway. Therefore, it plays an important role in carcinogenesis. From recent studies, variation in the gene is known to be involved in gastric cancer. AMPK α might induce anti-inflammatory response in adipose tissue, upon its activation resulting in a beneficial effect in obesity. Decreased cellular ATP or AMP is known to stimulate AMPK enzyme. Activated AMPK stimulates pathways such as mitochondrial biogenesis, glucose transport in muscle and mitochondrial biogenesis, in order to replenish cellular ATP. On the other hand, it prevents anabolic pathways that consume ATP including biosynthesis of fatty acid and cholesterol as well as adipogenesis. The AMPK signaling pathway is generally stimulated in response to stress, energy deficiency and/or oxygen inadequacy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-AMPK α antibody detects endogenous levels of total AMPK α protein. |
| General description: | The AMPK α (protein kinase AMP-activated catalytic subunit α1) gene is located on human chromosome 5p13.1. The gene is evolutionarily conserved. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AMPK alpha. Immunogen Range: 140-189 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |