Anti-PKC α antibody produced in rabbit
SIGMA/SAB4502354 - affinity isolated antibody
Synonym: KPCA; PKC III; PKC-α; PKC-A; kinase PKC-α
Product Type: Chemical
Immunohistochemistry Anti-PKC α: Cat. No. SAB4502354: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-PKC α antibody. Cells on the left were treated with the Anti-PKC α antibody. Cells on the right (negative control) were treated with both Anti-PKC α antibody and the synthesized immunogen peptide.
Western Blotting Anti-PKC α: Cat. No. SAB4502354: Immunoblotting analysis of extracts from NIH-3T3 cells, treated with UV 15′, using Anti-PKC α antibody. The lane on the left was treated with the Anti-PKC α antibody. The lane on the right (negative control) was treated with both Anti-PKC α antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 76 kDa |
| NCBI accession no. | P17252   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P17252 |
| Biochem/physiol Actions: | PKC (protein kinase C) is known to mediate different cellular processes, such as transcription regulation, cell signaling, cell proliferation, immune responses and apoptosis. While PKC is also associated with the learning ability. Abnormal cell signaling induced by PKC is often observed in cancer. PKC α (protein kinase C α) being one of the isozymes of the PKC family, it catalyzes the phosphorylation of substrates with many peptides. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-PKC α Antibody detects endogenous levels of total PKC α protein. |
| General description: | PKC α (protein kinase C α) gene is mapped to 17q24.2. PKC α is expressed in heart, liver, brain and epithelium. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKC alpha. Immunogen Range: 606-655 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |