Anti-HDAC8 antibody produced in rabbit
SIGMA/SAB4502369 - affinity isolated antibody
Synonym: FKSG78; G-protein coupled receptor 95; GPR87; GPR95; Probable G-protein coupled receptor 87
MDL Number: MFCD02686596
Product Type: Chemical
Immunohistochemistry Anti-HDAC8: Cat. No. SAB4502369: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-HDAC8 antibody. Cells on the left were treated with the Anti-HDAC8 antibody. Cells on the right (negative control) were treated with both Anti-HDAC8 antibody and the synthesized immunogen peptide.
Western Blotting Anti-HDAC8: Cat. No. SAB4502369: Immunoblotting analysis of extracts from NIH-3T3/HeLa/Jurkat cells, , using Anti-HDAC8 antibody. The lane on the left was treated with the Anti-HDAC8 antibody. The lane on the right (negative control) was treated with both Anti-HDAC8 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 41 kDa |
| NCBI accession no. | Q9BY41   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9BY41 |
| Biochem/physiol Actions: | The HDAC8 (histone deacetylase 8) protein is significantly involved in cartilage development and matrix-specific gene transcription by catalyzing histone acetylation. HDAC8 has Zn2+-dependent hydrolase activity that deacetylates the acetyl lysine residues of both histone and non-histone proteins. Deacetylation of structural maintenance of chromosome protein 3(SMC3) by HDAC8 is necessary for recycling of cohesin complex during cell cycle. Mutation in the gene is observed in Cornelia de Lange syndrome which is characterised by mental retardation and facial deformity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-HDAC8 Antibody detects endogenous levels of total HDAC8 protein. |
| General description: | The HDAC8 (histone deacetylase 8) gene is mapped to human chromosome Xq13.1. The encoded protein belongs to the class 1 histone deacetylase family. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HDAC8. Immunogen Range: 5-54 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |