Anti-PKD1 antibody produced in rabbit
SIGMA/SAB4502371 - affinity isolated antibody
Synonym: KPCD1; PKC-mu; PKCM; PKD; PRKCM
Product Type: Chemical
Immunohistochemistry Anti-PKD1: Cat. No. SAB4502371: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-PKD1 antibody. Cells on the left were treated with the Anti-PKD1 antibody. Cells on the right (negative control) were treated with both Anti-PKD1 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-PKD1: Cat. No. SAB4502371: Immunofluorescence of A549 cells using Anti-PKD1 antibody. Cells on the left were treated with the Anti-PKD1 antibody. Cells on the right (negative control) were treated with both Anti-PKD1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-PKD1: Cat. No. SAB4502371: Immunoblotting analysis of extracts from HuvEc cells, treated with PMA 125ng/ml 30′, using Anti-PKD1 antibody. The lane on the left was treated with the Anti-PKD1 antibody. The lane on the right (negative control) was treated with both Anti-PKD1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 101 kDa |
| NCBI accession no. | Q15139   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 |
| Application: | Anti-PKD1 antibody produced in rabbit has been used in western blot analysis(1:500). |
| Biochem/physiol Actions: | Polycystic kidney disease 1/polycystin-1 (PKD1/PC1) plays a regulatory role in cell migration and cell proliferation through mammalian targets of rapamycin (mTOR) and Janus kinase (JAK) signals. PKD1 inhibits cell migration and in some situations, acts as a tumor suppressor protein in the lung cancer cell line (A549). Before birth, polycystin-1 is the most active protein in kidney cells, and it interacts and works with polycystin-2 (PKD2). Mutations in the PKD1 gene are responsible for 85 percent of polycystic kidney diseases (PKDs). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-PKD1 Antibody detects endogenous levels of total PKD1 protein. |
| General description: | Polycystic kidney disease 1/polycystin-1 (PKD1/PC1) gene comprises of 46 exons and spans approximately 52 kb of genomic sequence. Polycystin-1 (PC1) is expressed at high levels in the brain and moderately in the kidney in adult tissue. It is present in the primary cilium of most mammalian cells. PC1 is also located at focal adhesions, desmosomes, and adherens junctions in the plasma membrane. Anti-PKD1 antibody detects endogenous levels of total PKD1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu. Immunogen Range: 171-220 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |