Anti-PKC ζ antibody produced in rabbit
SIGMA/SAB4502380 - affinity isolated antibody
Synonym: KPCZ; PKC-ζ; PKC2; PRKCZ; Protein kinase C ζ type
Product Type: Chemical
Immunohistochemistry Anti-PKC ζ: Cat. No. SAB4502380: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-PKC ζ antibody. Cells on the left were treated with the Anti-PKC ζ antibody. Cells on the right (negative control) were treated with both Anti-PKC ζ antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-PKC ζ: Cat. No. SAB4502380: Immunofluorescence of NIH-3T3 cells using Anti-PKC ζ antibody. Cells on the left were treated with the Anti-PKC ζ antibody. Cells on the right (negative control) were treated with both Anti-PKC ζ antibody and the synthesized immunogen peptide.
Western Blotting Anti-PKC ζ: Cat. No. SAB4502380: Immunoblotting analysis of extracts from COS7 cells, treated with PMA 125ng/ml 30′, using Anti-PKC ζ antibody. The lane on the left was treated with the Anti-PKC ζ antibody. The lane on the right (negative control) was treated with both Anti-PKC ζ antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 67 kDa |
| NCBI accession no. | Q05513   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q05513 |
| Application: | Anti-PKC ϲ, C-Terminal antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Isoenzymes of protein kinase C (PKC) family play a vital role in downstream signaling and cell functions. PKC ϲ regulates adhesion and effector functions of human eosinophils stimulated with platelet-activating factor (PAF) or complement (C) 5a. PKC ϲ and PKC δ might act as a therapeutic target for decreasing eosinophilic inflammation in clinical conditions such as asthma. PKC ϲ is calcium–independent, and is activated by phospholipids including phosphatidic acid (PA), phosphatidylinositol triphosphates and ceramides. Experimental studies state that, PKC ϲ is involved in the mitogen-activated protein kinase (MAPK) cascade, transcriptional factor NF-κB (NF-κB) activation, ribosomal S6-protein kinase signaling, and cell polarity. PKC-ϲ mediates hypoxia-induced Na,K-ATPase endocytosis in the alveolar epithelial cells (AEC).4 PKC-ϲ functions as a potential biomarker for human prostate cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Protein kinase C (PKC) ϲ is an isoenzyme, which belongs to the atypical class of protein kinase C family. PKCϲ is encoded by the gene mapped to human chromosome 1p36.33-p36.2. PKC ϲ is expressed in human eosinophils. This protein is characterized with a PB1 (Phox and Bem1) domain in the N-terminal, a pseudosubstrate (PS) sequence, a C1 domain (or phorbol esters/diacylglycerol binding domain) of a single Cys-rich zinc-finger motif, and a kinase domain in the C-terminal. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKC zeta. Immunogen Range: 526-575 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |